Immunohistochemistry for c-Fos showed significantly fewer c-Fos immunopositive cells in the pIIIu in the mice administered D-Lys3-GHRP-6 (3.90 ± 0.50) when compared to saline-treated mice (22.23 ± 2.52) [F(1,25) = 41.15, p < 0.0001] but no difference was seen in the VTA or the Arc (Fig. 3).
