When considering multiple markers of spatial and temporal identity, hiPSC NPCs show considerable inter-cell heterogeneity. Across SZ patients and controls, most hiPSC NPCs are positive for the NPC markers NESTIN (NES) and SOX2 (Supplementary Figure 3A). βIII-tubulin staining is visible in all NPC populations but does not appear to vary between control and SZ (Supplementary Figure 3A). Markers of neural stem cells, such as VIMENTIN (VIM) and PAX6, and neuronal progenitors, such as TBR2, are expressed by control and SZ hiPSC NPCs (Supplementary Figure 3A). There was no detectable difference in the labeling of control and SZ hiPSC NPCs by the cell cycle marker Ki67 (Supplementary Figures 3A and B). Doubling time between control hiPSC forebrain NPCs (3.69±0.05 days) and SZ hiPSC forebrain NPCs (3.60±0.02 days) did not differ (P>0.5) (Supplementary Figure 3C). Similarly, using cell cycle analysis, the percentage of cells in G1 (62.3±0.2% and 61.0±0.1%, P>0.3), S (24.6±0.1 and 24.9±0.1, P>0.7) and G2 (6.5±0.03 and 6.5±0.02%, P>0.3) phases did not differ between control and SZ hiPSC forebrain NPCs (Supplementary Figure 3D). Regional patterning between NPC lines appears to
