the same exposure level (Figure 5M, a). Similarly, the significant upregulation of S100b transcript levels by decitabine with no change in the number of S100B+ cells could be explained by the increased intensity of S100B in decitabine-treated cells, when the images were taken at the same exposure time (Figure 5M, b). Taken together, these results suggest that decitabine has minimal effect on the differentiated number of neurons, astrocytes and oligodendrocytes under the described conditions. They further suggest that the detected changes in Mecp2 expression are not likely due to altered population of differentiating cell types.
