experiments showed that there was no significant change in the number of TUB III+, GFAP+, CNPase+, or MBP+ cells. However, we did not find any NEUN+, or S100B+ cells in the control or decitabine-treated populations at D2, probably because these cells are still in the early stages of differentiation (Figure 5J). In the D8 population, decitabine treatment led to insignificant changes in the transcript levels for all neuronal and oligodendrocyte markers compared to control untreated cells. Additionally, Gfap expression in decitabine-treated cells was downregulated 5.5-fold, whereas S100b expression was upregulated to a similar extent (6-fold) (Figure 5K). Quantification of differentiated neurons, astrocytes and oligodendrocytes at D8 by IF did not show any significant change in the cell-fate commitment of these cells (Figure 5L). However, reduced Gfap expression in decitabine-treated cells without any changes in the number of GFAP+ cells might be explained by the reduced intensity of GFAP staining relative to the control astrocytes, since the images were taken at the same exposure level (Figure 5M, a). Similarly, the significant upregulation of S100b transcript levels by decitabine with no change in the number of S100B+ cells could be explained by the increased intensity of S100B in decitabine-treated cells, when the
