In order to relate EtOH-induced methylomic alterations to transcriptional changes we correlated EtOH-induced methylation changes at the promoter regions (Fig. 4 A-C) and the CpG islands (Fig. 4D-F) to the changes in gene expression under three conditions: (a) differentiation (Fig. 4A and D), (b) EtOH treatment of undifferentiated hESCs (Fig. 4B and E), and (c) EtOH treatment of differentiated EBs (Fig. 4C and F). Cutoff was made by β value change >0.5 in DNA methylation and >2-fold in gene expression. From these correlations, we observed that the majority of expression and methylation correlations occurred not only during differentiation alone but also following EtOH treatment of both undifferentiated hESCs and EBs (Fig. 4G).
