to the observed global increases in methylation at promoter regions and CGIs (Figs. 2 & 3), these data demonstrate that while a greater number of genes were hypomethylated, the increases in methylation of a smaller number of genes on individual chromosomes was more than sufficient to produce overall increases in DNA methylation status. These data also underscored the differential effect of EtOH exposure on the methylation of individual hESC genes and, in turn, suggested likely differential influence of EtOH on transcriptional regulation of genes on different chromosomes.
