Two sets of freshly-frozen autopsy brain tissue samples were obtained from the New South Wales Brain Tissue Resource Centre (NSWBTRC) in Australia. Set 1 included 480 [8 regions × (30 cases + 30 controls)] postmortem tissue samples dissected from eight brain regions [amygdala (AMY), caudate nucleus (CN), cerebellum (CRB), hippocampus (HIP), nucleus accumbens (NAc), prefrontal cortex (PFC), putamen (PUT), and ventral tegmental area (VTA)] of 30 (21 male and 9 female) AUD and 30 (21 male and 9 female) control subjects (NSWBTRC approved project #: PID409). Set 2 included 360 [6 regions × (30 cases + 30 controls)] postmortem tissue samples dissected from six brain regions (AMY, CN, CRB, HIP, PFC, and PUT) of 30 (20 male and 10 female) AUD and 30 (20 male and 10 female) control subjects (NSWBTRC approved project #: PID191). All subjects were European Australians with no history of illicit drug abuse or major psychotic disorders (such as schizophrenia and bipolar disorder) according to the criteria in the Diagnostic and Statistical Manual of Mental Disorder 4th Edition (DSM-IV) [27]. Control subjects had no history of AUD.
