Total RNAs were isolated from 10 to 50 mg of postmortem brain tissue samples using the miRNeasy Mini Kit (QIAGEN, Valencia, CA, USA). RNA integrity number (RIN) and concentration were measured using the Agilent 2100 Bioanalyser with the Agilent RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). From the 480 Set 1 RNA samples, we selected 192 [from 8 brain regions of 12 AUD cases (6 males and 6 females) and 12 controls (6 males and 6 females)] with larger RINs (mean ± SD: 6.6 ± 1.3) for miRNA and mRNA transcriptome analysis. From the 360 Set 2 RNA samples, we selected 96 (from 6 brain regions of 8 male AUD cases and 8 male controls) with larger RINs (mean ± SD: 5.9 ± 1.4) for miRNA and mRNA transcriptome analysis. In both sets of selected RNA samples, cases and controls were matched by sex, age, RINs, and postmortem intervals (PMIs). Characteristics (including the amount of daily alcohol use, sex, age, PMIs, RINs, brain weight, brain pH, cerebral hemispheres, smoking, and liver disease) of these two sets of
