We performed differential gene expression analyses in thirteen major cell types in which there were greater than 50 cells of that type in more than 10 individuals with and 10 without AUD. We utilized a pseudobulk approach, summing counts across cells within each sample for each cell type, with sex, age, and ethnic origin as covariates. Samples were removed on a cell type-specific basis if the sample contained less than 50 cells of that cell type (See Supplementary Table 7 for a summary of the number of pseudobulk samples created for each cell type).
