55-hour old worms reared at 20°C were used. Several hundred worms were placed on unseeded plates containing the appropriate concentration of ethanol (prepared as for locomotion assays, see above) for 10 or 50 minutes. Worms were photographed for subsequent size analysis. Exactly 200 worms were picked from plates to a tube containing 20 μl ddH2O. Tubes were placed at −80°C until analysis. Worms were thawed on ice and ground in the tube with a pestle (Kontes pellet pestle, Fisher Scientific, USA). Worm homogenate was stored at −20°C.
