Chunk #11 — Methods — Internal Ethanol Concentration calculations — Exposure to ethanol

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Ethanol metabolism and osmolarity modify behavioral responses to ethanol in C. elegans.

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For 200 worms: Picking took an average of 1.5 minutes per condition. Picking itself caused a loss of between 0 μL and 0.25 μL of the water, evaporation in that time caused an undetectable loss of volume.