Photographs were analyzed using ImagePro Plus v6. Ten worms were used per strain per condition. A midline was drawn to determine the height, h. Three diameters were drawn, one at the vulva (the approximate center of the animal), one each at the approximate midpoint between the vulva and head or tail (Figure 2a), these lengths were averaged and used as the diameter, ½ of this length was the radius, r. Volume was determined as for a cylinder: volume = πr2h.
