Chunk #13 — Methods — Internal Ethanol Concentration calculations — Calculation of internal ethanol concentration

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Ethanol metabolism and osmolarity modify behavioral responses to ethanol in C. elegans.

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We calculated the internal ethanol concentration using the equation C1V1 = C2V2, where C1 = concentration of ethanol in homogenate, V1 = (20 μL + volume of 200 worms), V2 = volume of 200 worms. We solved for C2.