Homogenates were tested for ethanol concentration using a Hewlett Packard model 5890A gas chromatograph (GC) equipped with a flame ionization detector and 2 meter 5% Carbowax 20M 80/120 mesh packed column (Restek, Bellefonte, PA). Samples were kept frozen at −20°C until analysis. Injections were accomplished manually with a 10 μl gas-tight glass syringe. The GC parameters were: 5 μl injection volume, 7 minute sample run time, injector temperature 200°C, oven temperature isothermal 90°C, detector temperature 220°C, helium carrier gas flow rate 30 ml/min, hydrogen flame flow rate 25 ml/min and air flow rate 400 ml/min. Data were collected and analyzed by Clarity GC software (Apex Data Systems, Prague, CZ) using a linear regression analysis with no weighting. A 7 point calibration curve preceded the analysis of ethanol concentrations. Ethanol concentrations were calculated by the external standard method. Quality control ethanol standards preceded and followed each pair of samples. Accuracy of results was inferred if the assayed ethanol concentrations from quality control ethanol standards varied by no more than 10% from actual concentrations. All quality control standards met this criterion. If
