F (1, 29) = 26.450, P < 0.001; fertility condition ×stress interaction: F (1, 29) = 0.606, P = 0.443). Meanwhile, the novelty-suppressed feeding test (NSFT) was conducted on these mice and the results indicated that the CUS treated virgin and postpartum female mice displayed a significantly increased latency to eat (P = 0.042 and P = 0.001) (Fig. 1c, fertility condition: F (1, 27) = 1.149, P = 0.293; stress: F (1, 27) = 23.530, P < 0.001; fertility condition × stress interaction: F (1, 27) = 0.885, P = 0.355) and decreased food consumption during the 30-min period (P = 0.048 and P < 0.001) compared to none stress treated mice, and there were no obvious changes between virgin and postpartum female mice under none stress and CUS conditions (P = 0.898 and P = 0.388) (Fig. 1c, subgroups: F (3, 29) = 3.746, P = 0.0217; timepoints: F (2, 58) = 184.200, P < 0.001; subgroups × timepoints: F (6, 58) = 3.865, P = 0.003). By the way, increased behavioral despair, as shown by increased immobility in the forced swimming test (FST), was observed in the CUS treated mice as compared with non-stressed mice (P =
