timepoints: F (6, 58) = 3.865, P = 0.003). By the way, increased behavioral despair, as shown by increased immobility in the forced swimming test (FST), was observed in the CUS treated mice as compared with non-stressed mice (P = 0.017 and P < 0.001) We also did not found significant difference between virgin and postpartum female mice under none stress and CUS conditions (P = 0.235 and P = 0.672) (Fig. 1d, fertility condition: F (1, 29) = 0.359, P = 0.554; stress: F (1, 29) = 45.390, P < 0.001; fertility condition ×stress interaction: F (1, 29) = 4.749, P = 0.038). To exclude possible effects of non-specific motor activity on mobility, locomotor activity was tested and the results showed no significant difference of distance traveled (Fig. 1e, subgroups: F (3, 29) = 0.644, P = 0.593; timepoints: F (14, 406) = 35.790, P < 0.001; subgroups × timepoints: F (42, 406) = 1.016, P = 0.447) and total distance (P = 0.998 and P = 0.999) (subgroups: F (1, 29) = 0.034, P = 0.855; timepoints: F (1, 29) = 1.812, P = 0.189; subgroups × timepoints: F (1, 29) = 0.078, P = 0.782) in CUS
