We performed replication analysis in 1,342,778 samples provided by 23andMe (non-overlapping with the 23andMe samples included in our MDD-META), including 455,350 depression cases. 99% of our findings showed concordant direction of effect between these two very large and independent cohorts. Of 211 variants tested, 209 (99%) had the same direction of effect, 192 showed at least nominal significance p<0.05 (91%), 144 remained significant after correction for multiple comparisons p<0.05/211=2.37×10−4 (68%), and 81 were independently genome-wide significant p<5×10−8 (38%). Only 2 SNPs were discordant, both with p>0.05 (0.9%). This very strong replication indicates the consistency of the findings we report herein.
