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Chunk #10 — Methods — RNA sequencing

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Transcriptome alterations are enriched for synapse-associated genes in the striatum of subjects with obsessive-compulsive disorder.
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correlations over genes. Correlation was high between BA11 and BA47, 0.71, compared to others (0.16–0.25; Fig. S2). Thus, data for BA11 and BA47 were treated as a single brain region termed “OFC” by averaging expression, per gene and subject, over BA11 and BA47. In this process, we noted one male OCD subject for whom the BA47 and nucleus accumbens samples had likely been inadvertently switched; this subject’s data were removed. After QC, 14,184 genes remained for analyses for all brain regions and 13,623, 13,889, and 13,756 for OFC, caudate, and NAcc, respectively.