Chunk #15 — Results — Kal7 is expressed in the major neuronal cell types in nucleus accumbens and dorsal striatum — Usage of promoters encoding full-length mouse Kalrn is region-specific
Full-length Kalirin promoter usage was assessed by real-time Q-PCR; forward primers located in each initiation exon were paired with a common reverse primer located in exon 3 (Figure 3A). In addition to nucleus accumbens, RNA from prefrontal cortex and hippocampus was analyzed to look for region-specific promoter usage. Although PCR products of the expected size were detected for all four putative full-length promoters, Kalrn promoters B and C accounted for over 95% of the full-length Kalirin transcripts in the adult mouse brain (Figure 3B); usage of promoters A and D was detectable in all three brain regions, but was much less common. The promoter usage patterns for hippocampus and nucleus accumbens were similar to each other, but differed from the pattern observed in the prefrontal cortex. Full-length Kalrn promoters B and C were used equally in the prefrontal cortex, while promoter C was the major promoter in the hippocampus and nucleus accumbens.
