hESC lines were cultured in fibroblast medium without FGF-2 containing DMEM, 10% FBS, 1 mM L-glutamine, 100 uM MEM non-essential amino acids, and 0.1 mM beta-mercaptoethanol, for a week. Cells were passaged three times using 0.25% trypsin and then sorted for hThy1+/hTRA-1-81− populations. Sorted fibroblast-like cells were plated, passaged one more time, and then reprogrammed by using CytoTune®-iPS Sendai Reprogramming Kit (Invitrogen) following manufacturer’s instructions.
