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Chunk #14 — Materials and Methods — MRI Data Acquisition

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Initial evidence that OPRM1 genotype moderates ventral and dorsal striatum functional connectivity during alcohol cues.
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Neuroimaging was conducted using a 3 Tesla Siemens Trio MRI scanner, at the UCLA Ahmanson-Lovelace Brain Mapping Center. The protocol began with initial structural scans followed by a series of four functional runs, including the alcohol-cue exposure task, a stop signal task, a delay-discounting task, and a risky decision-making task (results from the latter three tasks will be reported elsewhere). A T2-weighted, high resolution, matched-bandwidth, anatomical scan (MBW) and a magnetization-prepared rapid-acquisition gradient echo (MPRAGE) were acquired for each subject to enable registration (TR, 1.9s; TE, 2.26ms; FOV, 250mm; matrix, 256×256; sagittal plane; slice thickness, 1mm; 176 slices). The orientation for MBW and echoplanar image (EPI) scans was oblique axial to maximize brain coverage. The alcohol taste cues scan included 184 functional T2*-weighted EPIs (TR, 2s; TE, 30ms; flip angle, 90°; FOV, 192mm; matrix, 64 × 64; voxel size, 3 × 3 × 4mm3; slice thickness, 4mm; 34 slices). The first six volumes collected were discarded to allow for T1 equilibrium effects.