Following behavioral testing and sacrifice, whole mouse skulls with brain intact were scanned at the UNC Biomedical Research Imaging Center (BRIC) on a 9.4 Tesla Bruker BioSpec spectrometer (Bruker Biospin Inc., Billerica, MA). High-resolution MRI images (0.12mm × 0.12mm × 0.12mm) were acquired using the following diffusion weighted 3D RARE sequence: TR=0.7s, TEeff =23.662ms, Rare Factor = 3, RARE echo spacing = 11.3067ms, diffusion gradient time δ =6ms, diffusion gradient separation Δ = 12.422, b value – 1000s/mm2, matrix size = 200×125×80, FOV=24.0mm × 15mm × 9.6mm. Total scan time was 15 hours per animal. Each head was scanned individually.
