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Chunk #7 — Materials and methods — Drugs — Study 1: effects of clonidine treatment on alcohol intake

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The α2-adrenergic receptor agonist, clonidine, reduces alcohol drinking in alcohol-preferring (P) rats.
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The alcohol solution was prepared by diluting 95% alcohol (ethanol; Decon Laboratories Inc., King of Prussia, PA) with distilled, deionized water to make a 15% (v/v) solution. Alcohol (15% v/v) and water were presented in calibrated glass drinking tubes, with positions of the tubes alternated daily to control for potential side preferences. Daily fluid intakes were recorded to the nearest mL. Alcohol intake was converted from mL alcohol/kg BW to g alcohol/kg BW.