Live‐staining was performed with the addition of 150 μl of Ο4 antibody (1:300, R&D Systems) or platelet‐derived growth factor alpha (PDGFRα) (1:200 Cell Signaling, MA, USA) in culture media to 100 μl of culture media left in each 24‐well plate. Cells were incubated at 37°C for 40 minutes after which coverslips were washed with media and stained with secondary antibody diluted in media (1:1,000). Cells were incubated at 37°C for a further 30 minutes, washed, and then assessed electrophysiologically.
