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Chunk #8 — Material and Methods — Electrophysiology

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Maturation and electrophysiological properties of human pluripotent stem cell-derived oligodendrocytes.
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The whole‐cell patch configuration was used to record macroscopic currents as described previously 20, 21. Reported potential values are corrected for liquid junction potential (+14 mV). Responses to α‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazolepropionic acid (AMPA) and N‐methyl‐D‐aspartate (NMDA) were recorded at –74 mV and responses to gamma‐aminobutyric acid (GABA) and glycine at –14 mV. Current and voltage measurements were typically low‐pass filtered online at 2 kHz, digitized at 10 kHz, and recorded to computer using the WinEDR V2 7.6 Electrophysiology Data Recorder (J. Dempster, Department of Physiology and Pharmacology, University of Strathclyde, UK; www.strath.ac.uk/Departments/PhysPharm/).