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Chunk #2 — Materials and methods — Human post-mortem brain tissue collection and dissection

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Quality control parameters on a large dataset of regionally dissected human control brains for whole genome expression studies.
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An additional 36 brains originating from neuropathologically confirmed control Caucasian individuals were collected by the Sun Health Research Institute (SHRI) an affiliate of Sun Health Corporation, USA (Beach et al. 2008). In this case, whole brains were removed as fresh tissue at autopsy and brain coronal slices were frozen. Anatomical regions of interest were sampled from brain coronal slices on dry ice. The time interval from the removal of the brain at the mortuary to the completion of the dissection and placement of samples within the storage freezer ranged from 2.5 to 4 h. The parietal cortex, hypothalamus and spinal cord regions were not available for these samples. The total number of samples processed was 476 as again, some regions were not available from some brains. Detailed phenotypic information is described in Table 1.