Statistical analyses were performed using GraphPad Prism 5 (San Diego, CA). All studies used at least three different breeding rounds, with the reported n corresponding to the number of dams. Gene expression data was analyzed using the Student's 2-tailed t test; n = 6 per group for microarray analysis and for qRT-PCR validation, with separate cohorts of animals used for each analysis. Statistical significance was set at a 95% confidence interval.
