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Chunk #14 — Materials and methods — Microarray qPCR analysis and validation

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Prenatal alcohol exposure alters expression of neurogenesis-related genes in an ex vivo cell culture model.
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were also run with each primer set. The dissociated curves were evaluated. Results were assessed using the comparative CT method (ΔΔCT) and are expressed as relative fold change for each gene comparing PAE to control. At least four different cell cultures (generated from different dams, each with 8–9 embryos) were used for each group, n = 4–6.