tested for their effect on ASC multimerization upon immune stimulation by expressing the WT or lysine mutant constructs in Asc-/- BMDMs. Immunofluorescent detection of ASC with an anti-ASC antibody shows the two lysines are important for the formation of ASC specks upon stimulation with LPS and nigericin (Fig. 8d−g and Supplemental Fig. 7f). Immunofluorescent detection of ASC expression in BMDMs verifies that mutation of the lysine residues around position 21 didn’t cause retention of ASC in the nucleus, as previously identified for PML-dependent control of ASC (Fig. 8h and Supplemental Fig. 7g)7.
