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Chunk #16 — 2. Methods — 2.1. Sample and Measures

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Association of Polygenic Liability for Alcohol Dependence and EEG Connectivity in Adolescence and Young Adulthood.
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EEG recording and processing have been detailed previously [24]. Briefly, resting (eyes-closed) EEG was recorded for 4.25 min; a continuous interval of 256 s was analyzed. Each subject wore a fitted electrode cap (Electro-Cap International Inc.; Eaton, OH) using the 19-channel montage as specified according to the 10–20 International system (FP1, FP2, F7, F3, FZ, F4, F8, T7, C3, CZ, C4, T8, P7, P3, PZ, P4, P8, O1, O2). The nose served as reference and the ground electrode was placed on the forehead. Electrode impedances were always maintained below 5 KOhm. EEG was recorded with the subjects seated comfortably in a dimly lit sound-attenuated temperature-regulated booth (Industrial Acoustics Company; Bronx, NY, USA). They were instructed to keep their eyes closed and remain relaxed, but not to fall asleep. Electrical activity was amplified 10,000 times by Neuroscan amplifiers, with a bandpass between 0.02 Hz to 100 Hz and recorded using the Neuroscan software system (Compumedics Limited; El Paso, TX, USA) running on i86 PCs. EEG procedures were identical at all collection sites. Further details regarding the EEG coherence data processing is