The regulation of reward feeding or palatable food consumption by DA neurons has been well studied10. Moreover, central deletion of FoxO1 has been shown to enhance anorexigenic effect23202122. This led us to investigate whether FoxO1 in DA neurons is involved in the regulation of reward-related feeding and palatable food consumption. To address this question, sucrose preference tests were performed in FoxO1 KODAT mice. While 1 and 5% sucrose showed no difference between genotypes (Supplementary Fig. 3d), FoxO1 KODAT mice showed increased sucrose intake in 2% sucrose compared with WT littermates, which was not evident in fasted condition (Fig. 4e). These results indicated that FoxO1 in DA neurons seems to be involved in the regulation of specific feeding behaviours such as palatable food consumption. We next examined the contribution of food reward aspect to HFD consumption in the KO mice. However, FoxO1 KODAT mice showed no difference in HFD intake compared with WT littermates (Fig. 4f). Taken together, these results suggest that the difference in reward-related feeding in FoxO1 KODAT mice might not affect the homoeostatic feeding as the total calories
