The gene specific library had a significantly greater number (152-fold) of GABAB1 specific mapped reads than the whole transcriptome library though they have similar total mapped reads (Figure 2; Table S2 in Supplement 1). In addition, a significantly greater number of splice junctions were identified in the gene specific library (Figure 2). It identified 1,076 reads that contained GABAB1 specific splice junctions compared to only 18 reads from the whole transcriptome library. A greater number of mapped reads was observed on the 3’ end of the gene specific library (Figure 2A). The distribution likely resulted from gene specific reverse transcription or postmortem RNA degradation.
