Gene specific library was designed for RNA-seq to maximize splice junction identification in the GABAB1 gene. Current approaches to RNA-seq library construction often utilize random primers or oligo(dT) primers for reverse transcription (22-26). However, these approaches do not generate enough mapped reads to find rare splice junctions from a single RNA-seq run. To provide a large population of gene specific reads, we constructed the library using gene specific reverse transcription (Figure 1).
