We chose the 10 highest and lowest scoring strains for the Dist and ΔDist metrics as the strongest candidate ethanol behavioral mutants (Table 1). Where more than 1 lesion in or near a gene resulted in similar behavioral scores, we chose 1 strain as representative for that gene. Additionally, some strains showed strong behavioral scores for both Dist and ΔDist. Ethanol absorption was unaffected in these strains, indicating that the observed behavioral alterations were not due to altered ethanol pharmacokinetics (Table 1). While we did not explicitly screen for ethanol sedation sensitivity and sedation tolerance mutants, 11 additional strains showed strong effects for either one or both measures (Table S6). Here we briefly discuss a subset of the strongest Dist and ΔDist mutants, followed by a more in-depth analysis of 4 mutant strains in which the genetic lesion completely disrupts the associated gene.
