Selection details are found in Colville et al. (2017). Briefly, HS-CC founders (Iancu et al., 2010) were selected for breeding based on their preference for 10% ethanol vs. water. Beginning with 200 founders, the 20 males and 20 females with the highest preference values were paired, with brother-sister matings avoided, to create a “High” preference line; similarly, the 40 mice with the lowest preference scores were paired to create a “Low” line. ∼200 pups from each generation were weaned and tested at adulthood as above for three subsequent generations; active selection concluded at S3. S4 alcohol-naive pups were used for genetic analyses.
