iPSCs were generated from fibroblasts obtained using a skin punch biopsies of the inner, upper arm from 24 participants enrolled in studies at UCONN Health (UC, Farmington, CT). Fibroblasts from 11 control subjects (CTLs) were generated from non-alcoholic participants enrolled in a study examining the subjective effects of acute alcohol intoxication (Covault et al. 2014, Milivojevic et al. 2014). Fibroblasts from 13 individuals with a DSM-IV diagnosis of alcohol dependence (ADs) were generated from participants enrolled in a pharmacological treatment study (Kranzler et al. 2014). The average age of CTLs was 30.5 years while that of ADs was 46.1 years. All CTLs were male, while the ADs included 10 males and 3 females. The control sample was limited to males due to restrictions of enrollment in the clinical study the fibroblast samples were obtained (Covault et al. 2014). Biopsy samples were minced and cultured in Dulbecco’s modified eagles medium (DMEM, Thermo Fisher Scientific) supplemented with 20% fetal bovine serum (FBS, Thermo Fisher Scientific), 1x non-essential amino acids (Thermo Fisher Scientific) and 1x penicillin/streptomycin (Thermo Fisher Scientific). Fibroblast cultures were expanded and passaged using trypsin (Thermo Fisher Scientific) prior to being frozen or sent for reprogramming.
