The UC Stem Cell Core (Farmington, CT) reprogrammed fibroblasts to pluripotency using a retrovirus to express five factors (OCT4, SOX2, KLF4, c-MYC, and LIN28) or a sendai virus to express four factors (OCT4, SOX2, KLF4, and c-MYC), reflecting a change in reprogramming methodology over the course of the project. Two to four weeks after viral transduction, multiple pluripotent stem cell colonies for each subject were selected and expanded as individual iPSC lines. Expression of pluripotency markers by iPSC cells was verified by immunocytochemistry for SSEA-3/4 and NANOG by the UC Stem Cell Core. The total sample for the current experiments consisted of 35 iPSC lines generated from the 24 subjects, with 2 (or 3) clones examined from 10 subjects (6 CTLs and 4 ADs) and 1 clone examined from 14 subjects (5 CTLs and 9 ADs). In total, 5 of the 35 lines examined in this report were from female donors.
