To simulate the voltage dependence of STOCs using the measured spatial relationship between BK channels and RYRs, we performed STOC simulations clamped at the different voltages. Fig. S3 displays the time course of Po for each BK punctum marked in Fig. 5 A at different voltages. Using the same calculation as in Fig. 7 A, we can estimate STOCs for that RYR2 cluster at each voltage. Fig. 8 A shows STOC events at different voltages from 25 RYR2 clusters. Fig. 8 (B and C) displays the relationship of three STOC parameters to voltage from 862 pairs of RYR2s and BK channels from six cells. Strikingly, all of the three aforementioned parameters in simulated STOCs show the same relationship to the voltage as STOCs recorded experimentally.
