Each line was cultured and plated as previously described 67. Briefly, 96-well plates were coated with three concentrations of Fibronectin in alternated columns in a randomised fashion. Cell lines were seeded also in rows in a randomised fashion. 3,000 cells were plated and fixed after 24 hours. EdU was incorporated 30 minutes before fixation. Plates were then fixed and stained with DAPI and cell mask and EdU staining. Images were acquired using the Operetta (Perkin Elmer) high content device. Using the Harmony software, measurements were derived for each cell. Measurements included intensity features (DAPI, EdU), morphology features (cell area, cell roundness, cell width to length ratio, nucleus area, nucleus roundness, nucleus width to length ratio) and context features related with cell adhesion properties (number of cells per clump). Processing quantification and normalization of data was performed as previously described 67. The Cellomics fluorescence imaging data was used to quantify the fraction of cells expressing each protein marker independently. In the absence of co-staining information we sought to use the marginal fractions of cells expressing each marker to calculate lower and
