Chunk #70 — STAR* METHODS — QUANTIFICATION AND STATISTICAL ANALYSIS — Enrichment analysis using brain developmental, regional, and cell-type-specific data
Single cell expression profiles from the adult brain (Darmanis et al., 2015) were used to identify cell-type specificity of candidate genes. Single cell expression values were log-transformed and centered using the mean expression values. Average centered expression values for candidate genes were calculated in each cell. Cells were then grouped into cell clusters (neurons, astrocytes, microglia, oligodendrocytes, OPC, and endothelial cells), and a relative expression level for a given cell cluster was calculated by a scale function in R.
