Chunk #69 — STAR* METHODS — QUANTIFICATION AND STATISTICAL ANALYSIS — Enrichment analysis using brain developmental, regional, and cell-type-specific data
To obtain genes that show cortical regional enrichment (e.g. frontal cortical enrichment), we computed t-statistics for each gene for a specific cortical region (e.g. frontal cortex) versus all other cortical regions (e.g. parietal cortex, temporal cortex, and occipital cortex, Kang et al. 2011). The top 5% of genes that show heightened expression patterns for each cortical region were selected as region-specific genes. These genes were then overlapped with candidate genes by Fisher’s exact test to measure cortex regional enrichment.
