Behavioral studies were performed during the light cycle between 7:00 a.m. and 7:00 p.m. Before experiments, the animals were handled for 5 min every day for 2 weeks. On the day of testing, to habituate subjects, mice were left in their home cages in the room used for the experiment at least 1 hour prior to the onset of the study. To minimize olfactory cues, each apparatus was thoroughly cleaned with 10% ethanol after each animal. Three cohorts of mice were tested and the results averaged. Total number of animals per genotype: 2N:Kcnj6++, n = 19; Ts65Dn:Kcnj6+++, n = 12; and Ts65Dn:Kcnj6++−, n = 14. All behavioral tests and procedures were performed by personnel blinded to genotype.
