objectives. The zoom was set between 1× and 2× according to the cell size, with a pinhole of 34 μm and a speed of 1.58 μs per pixel. The confocal laser intensity was set to 2 and the gain was adjusted per experiment for optimal result. The dimensions were set to 512 × 512 pixels with 4 times averaging. For each condition 5 pictures were taken and all experiments were repeated 3 times. A script was written in Fiji 1.49 (ImageJ) that automatically calculated the surface area of the KCNAB2 staining, DAPI staining (nuclear surface area) and Phalliodin staining (cytoplasmic surface area). The percentage of nuclear and cytoplasmic surface area that contained staining was calculated. The time points were separately compared to the control using an unpaired samples t-test.
