Additional file 1: Figure S1. Ethanol preference and consumption is unaffected by CVC treatment. Mice were housed singly and received daily injection of CVC. Two-bottle choice of water or alcohol in drinking water at increasing concentrations (3, 6, 9, and 12%) for 4 days each was provided ad libitum. Consumption was measured, and mice were provided freshwater and alcohol daily. A) The amount of ethanol consumed at each concentration was normalized to mouse body weight (BW). B) Alcohol preference was determined as a ratio of the volume of alcohol consumed to the total liquid volume consumed per day. C) Total fluid intake (water plus alcohol) was measured and normalized to the respective body weight of each mouse. Data are mean +/- SEM, n = 8 mice/group. Two-way ANOVA with Bonferroni correction results are described in the text.
