EST-abundance advantages over other tissues. We also observe a high level of AS in the human liver, a tissue with much lower EST coverage, where higher levels of AS have been previously reported in cancerous cells [33,34]. Human muscle, uterus, breast, stomach and pancreas had the lowest levels of AS genes in this analysis (less than 25% of genes). Lowering the minimum EST count for inclusion in this analysis from 20 to 10 ESTs, and sampling 10 (out of 10 or more) ESTs to represent each gene in each tissue, did not alter the results qualitatively (data not shown).
