Alternatively spliced genes in this analysis exhibited on average between one and two distinct AS exons. Analyzing the different types of AS events separately, we found that the human brain and testis had the highest levels of skipped exons, with more than 20% of genes containing SEs (Figure 1b). The high level of skipped exons observed in the brain is consistent with previous analyses [29,30,32]. At the other extreme, the human ovary, muscle, uterus and liver had the lowest levels of skipped exons (about 10% of genes).