DBTSS and JASPAR indicated that the GTSE1 promoter contains three putative binding sites for YY2, and the ChIP assay showed that YY2 could directly bind to the GTSE1 promoter at putative binding sites 2 and 3, whereas there were no interactions observed in the NC or at putative binding site 1 (Figure 7D). Similarly, both GTSE1 mRNA and protein levels were decreased in the LINC00665+ + YY2− group compared with those of the LINC00665+ + YY2+ group, and the expression levels were higher in the latter group (Figures 7E and 7F). These results indicated that both MTF1 and YY2 participate in the transcriptional promotion of GTSE1.
