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Chunk #9 — Materials and Methods — Chronic Intermittent

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Gene expression in brain and liver produced by three different regimens of alcohol consumption in mice: comparison with immune activation.
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total length of the study, including drinking and non-drinking days, was 57 days. CI animals were maintained on a 12∶12 light/dark light cycle (lights on at 07∶00 AM). Control animals received only water. Data from all animals was used for ethanol consumption analyses. For array analysis, one treated animal was omitted (chosen at random) from microarray experiments in order to maintain continuity of sample sizes across treatment paradigms.