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Chunk #44 — ONLINE METHODS — DNA extraction, genotyping, imputation and cnV calling

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Genetic variability in the regulation of gene expression in ten regions of the human brain.
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Genomic DNA was extracted from sub-dissected samples (100–200 mg) of human postmortem brain tissue using Qiagen’s DNeasy Blood & Tissue Kit (Qiagen, UK). All samples were genotyped on the Illumina Infinium Omni1-Quad BeadChip and on the Immunochip, a custom genotyping array designed for the fine mapping of autoimmune disorders1,52. The BeadChips were scanned using an iScan (Illumina, USA) with an AutoLoader (Illumina, USA). GenomeStudio v.1.8.X (Illumina, USA) was used for analyzing the data and generating SNP calls.