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Chunk #37 — Methods — DNA amplification

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Combined analysis of circulating β-endorphin with gene polymorphisms in OPRM1, CACNAD2 and ABCB1 reveals correlation with pain, opioid sensitivity and opioid-related side effects.
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The Magtration 12GC system (Precision System Science, Chiba, Japan) and the Magazorb® DNA Common Kit-200 (PSS, Chiba, Japan) were used for the total genomic DNA preparation from the whole blood samples. The DNA-fragments containing the SNP site was amplified in a reaction mixture on Mastercycler Ep Gradient S (Eppendorf AG, Germany).